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1 Pantha, K.; Acharya, K.; Mohapatra, S.; Khanal, S.; Amatya, N.; Ospina-Betancourth, C.; Butte, G.; Shrestha, S. D.; Rajbhandari, P.; Werner, D. 2021. Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing. npj Clean Water, 4:12. [doi: https://doi.org/10.1038/s41545-021-00099-1]
(Location: IWMI HQ Call no: e-copy only Record No: H050276)
(2.02 MB) (2.02 MB)
A suitcase laboratory was used for 16S rRNA amplicon sequencing to assess microbial water quality in the holy Bagmati River, Kathmandu, Nepal. SourceTracker analysis and Volcano plots revealed that microbial communities in the downstream part of the river were mainly contributed by untreated sewage. Seasonal variability in the sewage microbiome was reflected in the downstream river water quality. The bacterial genera Acidovorax, Geobacillus and Caulobacter predominated in the upstream sites, while genera containing putative human pathogens and gut bacteria, such as Clostridium, Prevotella, Arcobacter, Lactobacillus, Enterococcus and Streptococcus become prominent in the downstream sites. Marker gene qPCR assays for total bacteria, total coliforms, Human E. coli, Arcobacter butzleri and Vibrio cholerae confirmed the sequencing data trends. Even though basic sanitation provision is nowadays near universal in Nepal, our findings show how inadequate wastewater management may turn an urban river into an open sewer, which poses a public health risk.
(Location: IWMI HQ Call no: e-copy only Record No: H050398)
(4.00 MB) (4.00 MB)
The hydrological response to climate change in mountainous basins manifests itself at varying spatial and temporal scales, ranging from catchment to large river basin scale and from sub-daily to decade and century scale. To robustly assess the 21st century climate change impact for hydrology in entire High Mountain Asia (HMA) at a wide range of scales, we use a high resolution cryospheric-hydrological model covering 15 upstream HMA basins to quantify the compound effects of future changes in precipitation and temperature based on the range of climate change projections in the Coupled Model Intercomparison Project Phase 6 climate model ensemble. Our analysis reveals contrasting responses for HMA's rivers, dictated by their hydrological regimes. At the seasonal scale, the earlier onset of melting causes a shift in the magnitude and peak of water availability, to earlier in the year. At the decade to century scale, after an initial increase, the glacier melt declines by the mid or end of the century except for the Tarim river basin, where it continues to increase. Despite a large variability in hydrological regimes across HMA's rivers, our results indicate relatively consistent climate change responses across HMA in terms of total water availability at decadal time scales. Although total water availability increases for the headwaters, changes in seasonality and magnitude may diverge widely between basins and need to be addressed while adapting to future changes in a region where food security, energy security as well as biodiversity, and the livelihoods of many depend on water from HMA.
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